MACROH2A1 and steatosis: To determine whether protein expression levels of macroH2A1.1 and macroH2A1.2 were altered in the context of steatosis or HCC, a histone extraction protocol was applied to the livers of mice fed a ND, fed with HF or injected with DEN and fed with HF (HF/DEN), followed by immunoblotting analysis.