To model the tumor microenvironment of ovarian cancer in vivo, we used spheroids of HeyA8-CXCR4-CBRN/Ar-CBC cells combined with equal numbers of HeyA8-CXCL12-GL cells, reproducing human ovarian cancer in which tumor cells secrete CXCL12 and/or express CXCR4. This evidence concerns the gene CXCL12 and ovarian cancer.