Our study has conclusively demonstrated for the first time that (1) RHOA is upregulated in both PCa specimens and cell lines derived from AA patients compared to their corresponding CA counterparts, (2) AR can directly bind to the RHOA promoter, (3) AR binding to the RHOA promoter is higher in AA compared to CA PCa cell lines, (4) DHT can further stimulate AR binding to the RHOA promoter in AA PCa cell lines, and (5) DHT can stimulate invasion of AA PCa cells in a RHOA-dependent manner. Here, RHOA is linked to posterior cortical atrophy.