A safer way we suggest to study the role of Nrf2 in insulin resistance in vitro is the use of primary cultures of hepatocytes from WT and Nrf2-KO mice and induction of insulin resistance with treatment with tumor necrosis factor-α or dexamethasone as described previously [21] followed by estimation of insulin signaling between the different genotypes. The gene discussed is NFE2L2; the disease is Insulin resistance.