Furthermore, to verify whether mGPC3-1 peptide-specific CTLs were induced in STAM mice C57BL/6N-NASH, a mGPC3-1 peptide vaccine was given and an IFN-γ ELISPOT assay was performed using RMA-S pulsed with mGPC3-1 peptide or non-pulsed RMA-S. Here, IFNG is linked to metabolic dysfunction-associated steatohepatitis.