Control Saos-2 cells developed a primary tumor at the site of implantation in all cases (2.57 mm2 ± 1.37; n = 12 Fig. 6a; A, B, and G), whereas the majority of Saos-2 Snail2 knock-down cells did not form a tumor (n = 7/12), and those that did formed markedly smaller and less dense tumors (1.01 mm2 ± 0.74; P < 0.001) (Fig. 6a; D, E, and G). This evidence concerns the gene SNAI2 and neoplasm.