Table S1 summarizes the results of cell signaling studies. In human RCC cell lines, Ku0063794 inhibited the activity of both mTORC1 and mTORC2, while temsirolimus activity was generally limited to mTORC1. Our study suggests that phosphorylation of mTOR at Ser2448 and Ser2481 is primary regulated by mTORC2 since phosphorylation was strongly inhibition by Ku0063794 but not temsirolimus. However, prior reports do not firmly assign these phosphorylation sites to mTORC2 [4]. This evidence concerns the gene MTOR and renal cell carcinoma.