Lower levels of Capn4 mRNA and protein expression were detected in the peritumor samples compared to the ICC samples (mRNA: 0.66±0.11 vs. 0.97±0.15, p = 0.003; protein: 1.10±0.08 vs. 2.54±0.17, p<0.001; Figs. 2A, 2B and 2C). The gene discussed is CAPNS1; the disease is intrahepatic cholangiocarcinoma.