Figure 4 shows the localization of anti-CD4, anti-CD8 and anti-CD56 antibodies coupled with ThPOK staining in samples of NM, MA, and CRC. The colocalization image was used to calculate the overlap coefficient according to Manders [32]. We observed interesting changes in the colocalization levels during neoplastic progression (Figure 4). The gene discussed is NCAM1; the disease is colorectal carcinoma.