INS and osteoporosis: Together, these results demonstrate that the long-term calorie restriction protocol performed here delays the onset of age-associated pathologies such as glucose intolerance, osteoporosis, and impaired neuromuscular coordination both in WT and TgTERT mice, although in some assays the effects tended to be of a greater magnitude in the TgTERT cohorts (i.e. insulin levels or tightrope test).