In the present study, We tried to explore the role of Notch2 in astrocytic gliomas by upregulation of Notch2 expression with transfection of Notch2 plasmid, and found that enforced overexpression of Notch2 in glioma cells was identically associated with the inhibition of cell proliferation, arrest of cell cycle, reduction of invasiveness of tumor cells and induction of cell apoptosis as shown by in vitro study. Here, NOTCH2 is linked to central nervous system cancer.