EGFR/PI3K/AKT signaling pathway plays a major oncogenic role in GBM, we measured the activity of EGF pathway after intervening the Notch signaling pathway, and found that Notch2 ectopic expression downregulated the EGFR expression as well as its downstream signaling proteins, including PI3K, p-AKT, which were essential to cell survival and proliferation in gliomas, also, affected the expression of protein relevant to cell invasion and apoptosis, such as MMP2, MMP9, Bcl-2 and Caspase-3. The gene discussed is EGFR; the disease is central nervous system cancer.