The utility of MethyLight for DNA methylation-based biomarker has been demonstrated by numerous studies, including the methylation of GSTP1, APC, TGFβ2, HOXD3, MLH1, dickkopf homolog 1(DKK1), and secreted frizzled-related protein 1 (SFRP1), which has been shown to be detected in prostate and colon cancers [43, 119–122]. Here, SFRP1 is linked to malignant colon neoplasm.