ACTA1 and neoplasm: Interestingly, in sections and locations with α-SMA-positive CAF-like cells, we also identified delicate, spindle-shaped cells at the periphery of the invading tumor islands or closely surrounding them that were either positive to both cytokeratin and twist in the HSC-3-myoma monocultures or, alternatively, only to twist in the HSC-3-CaDEC12-myoma cocultures (Fig. 3l–m).