There are a few limitations in this primary study: increasing the number of samples can helpful to indentify TGF-β1 roles in clinical assessment; further to investigate TGF-β1 gene’s function by interfering TGF-β1 expression in GC cells as well as in vivo assay will help to better explain its precise mechanism in tumor carcinogenesis. This evidence concerns the gene TGFB1 and gastric cancer.