HK2 and neoplasm: Fig. 6b shows representative images of HK-II IHC staining of the tumor. The staining pattern of pimonidazole-positive areas was similar to that of HK-II-positive areas. The typical IHC stainings of HK-II in Pimo+ and Pimo− are shown in Fig. 6c and d, respectively. The intensity and extent of HK-II staining were markedly greater in Pimo+ compared to Pimo−. The results of semiquantitative analysis of HK-II are summarized in Fig. 6e.