In summary, this study exhibit MIR of the wavelength in 3–5 μm can alter the organization of actin filament, microtubule and vinculin, and cause inhibition on cell cycle progression through activating ATM/ATR-p53-p21 axis in response to DNA damage, also the Cdc25C regulating pathway in parallel thus resulting in downregulation of dephosphorylation in CDK1 and cyclin B. In particular, our study shows the first evidence on the inhibitory effect of MIR in lung cancer cells and provides useful information for cancer therapy. This evidence concerns the gene ATM and lung cancer.