The qRT-PCR results were unable to detect significant diferences in the levels of transcripts except for Glypican-3 (GPC3), in which 85% of non-metastatic (p = 0.003) and all metastatic tumors (p = 0.005) evidenced a strong (approximately 10 fold and 12 fold respectively) sub-expression (Figure 1C). Here, GPC3 is linked to metastatic neoplasm.