BRAF and melanoma: This study compared the analytical performance of the CE-IVD marked and FDA-approved RT-PCR test with two other commercially available methods: bidirectional direct Sanger sequencing (“Sanger”) and the Applied Biosystems BRAF Mutation Analysis Reagents kit (“FA test”) for the detection of BRAF V600 mutations in formalin fixed paraffin embedded (FFPE) specimens of malignant melanoma.