For the in vitro screening of TKI-dependent proteome changes, we used a well-established murine CML model: Ba/F3 cells, an immortalized murine bone marrow-derived pro-B-cell line, are retroviraly transfected with either wild type (p210) or mutated BCR-ABL isoforms (M351T, T315I). Here, BCR is linked to chronic myelogenous leukemia, BCR-ABL1 positive.