Although differences in eosinophilia and IL-13 and mucin-5AC expression using this model failed to reach statistical significance, we believe this is likely due to the inherent heterogeneity of house dust mite extracts, which contain variable levels of LPS, chitins, and other allergens and can therefore elicit more complex immune responses than those to Der p 1 alone [21]. This evidence concerns the gene MUC5AC and Increased total eosinophil count.