AKT1 and acute myeloid leukemia: Together, by identifying an association between PI3K/AKT signaling activation and in vitro GO and calicheamicin-γ1 resistance, our data illustrate the capacity of SCNP assays to functionally characterize the integrity of biological pathways and signaling networks relevant to cellular responses to genotoxins such as calicheamicin-γ1-based antibody-drug conjugates in vitro in primary samples from patients with AML.