AKT1 and acute myeloid leukemia: As depicted in Figure 1, our SCNP assay focused on functional, quantitative measurements of DNA damage pathways as well as PI3K/AKT, MEK/ERK, and JAK/STAT signaling pathways in leukemic cells in response to in vitro stimulation of diagnostic specimens from 6 pediatric and 6 adult patients containing >78% blast cells in all cases (Table 1; AML-01– AML-12).