Similarly, analysis of the diagnostic and prognostic role of FOLR1 and FOLR3 in effusion cytology of ovarian cancer (n = 71), breast cancer (n = 10), and malignant mesothelioma (n = 10) using quantitative PCR and flow cytometry showed significantly higher concentrations of FOLR1 and FOLR3 in ovarian carcinoma samples compared to breast or mesothelioma. This evidence concerns the gene FOLR1 and ovarian cancer.