Tissue was collected in consistent ipsilateral and contralateral brain regions to measure iNOS protein expression at all time points, as shown in Figure 7A. Analysis of Western blots revealed no significant differences in iNOS expression in any ipsilateral region compared to the matching contralateral region in response to cerebral ischemia (Figure 7B) or during the first 8 and 24 h of reperfusion (Figure 7C and D). This evidence concerns the gene NOS2 and brain ischemia.