TNF and Granuloma: Were as staining of Δtgs1 Mtb cells from granulomas treated with IgG, as well as those from granulomas treated with anti-TNFα mAb showed that most of the cells were positive for Auramine-O (Fig. 7A), indicating that the Δtgs1 mutant Mtb cells accumulated less lipids as compared to WT Mtb. Staining of Δtgs1 C+ Mtb cells showed that most of the Δtgs1 C+ Mtb cells from granulomas treated with anti-TNFα mAb were positive for the Auramine-O stain indicating resuscitation, whereas most of the cells from granulomas treated with the control IgG, remained positive for Nile red indicating dormancy.