SATB1 and neoplasm: Next we analyzed SATB1 expression by immunohistochemical staining of the paraffin-embedded tissue sections consisting of 19 benign, 5 low-grade tumor (Gleason score 5–6), 10 median-grade tumor (Gleason score 7–8) and 5 high-grade tumors (Gleason score 9–10) in the tissue microarray (Figure 2).