SATB1 and neoplasm: Benign specimen (n = 15) exhibited a mean of 56.93 stained nuclei (range 37–79), low-grade tumors with Gleason score of 5–6 (n = 13) showed a mean of 90.54 stained nuclei (range 56–115); moderately differentiated tumors with Gleason score of 7–8 (n = 12) exhibited a mean of 146.33 stained nuclei (range 93–198); and high-grade tumor with Gleason score 9–10 (n = 11) showed a mean of 205.27 (158–298) stained nuclei for SATB1.