Interestingly, secreted BMI1 levels were not to be observed in all types of pancreatic (Kras-mutant PDE, E6E7-Ras and E6E7-Ras-st) and colon carcinoma cell lines (SW480, HCT116), but only in highly aggressive pancreatic cell lines AsPC1 (at very low levels; data not shown) and colon HT29 cells (data not shown). This evidence concerns the gene KRAS and colon carcinoma.