To ensure that the observed results were due to the loss of transcriptional activation by β-catenin and not mediated by protein turnover we concurrently conducted qt-PCR on MDA-MB-231 cells for HMGA2, proliferation markers and BIRC5 [Survivin, a well-known target of ICG-001 in colon cancer (Emami et al, 2004; Supporting Information Fig S5A)]. This evidence concerns the gene HMGA2 and colonic neoplasm.