Dellavance et al. evaluated over 10,000 ANA positive samples by HEp-2 IIF followed by a confirmatory immunoblot and reported that anti-DFS70 antibodies were common among ANA-positive individuals with no evidence of SARD and that among autoimmune patients with this autoantibody over a half had evidence of autoimmune thyroiditis [18]. This evidence concerns the gene BTG3 and autoimmune thyroid disease.