In the same way, the CD44 knockdown using shRNA lentivirus particles in BCSCs also induced their differentiation and down-regulated the expression levels of FASN and different stem cell-like markers, oncogenes and anti-apoptotic factors such as lymphoid enhancer-binding factor-1 (LEF-1), Myc, EGFR, mucin-1 and Bcl-2 and thereby sensitized these immature cancer cells to the anti-tumoral effect induced by doxorubicin [249, 267]. Here, LEF1 is linked to cancer.