Interestingly, quadrant IV (Axis1Axis2), which is not annotated by haematopoietic cells but correlated with AML and T-ALL, includes RUNX1, ERG, and MYB, which have well-established roles in AML and early haematopoietic differentiation including myeloid and T-lymphocyte lineages [40]–[43] (Fig. 1b). Here, RUNX1 is linked to acute lymphoblastic leukemia.