CD81 and infection: To determine the capability of the Huh-7.5/EG(4A/4B)GLuc cell line in antiviral-mediated neutralizations, cells were pre-treated with replication or entry inhibitors (IFN-α or anti-receptor antibodies [anti-CD81 or anti-SR-BI], respectively), or viruses were pre-incubated with the conformational anti-E2 antibody AR3A [28] prior to infection with JC1 virus at an MOI of 0.5 TCID50/cell.