Table 8 shows the top 5 genes in microarray one and microarray two respectively. In both microarray one and microarray two, ADH4 which encodes alcohol dehydrogenase 4 (class II), pi polypeptide showed very high rank indicating that ADH4 is the most significantly differentially expressed gene. More specifically, the expression of ADH4 was significantly up-regulated in NASH patients compared with normal controls. This evidence concerns the gene ADH4 and metabolic dysfunction-associated steatohepatitis.