IFNB1 and infection: To further define the ISGs expressed in an IRF-3, IRF-5, or IRF-7 dependent manner, we infected mDC and macrophages from WT, DKO, TKO, and Ifnar−/− mice with WNV-NY and measured the induction of Ifnb, Oas1a, Rsad2, and Cxcl10 mRNA at 24 hours after infection by quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Figure 3E and F).