Some studies have shown that GTP enzymes, such as Rho, Rac and Cdc42, through the downstream effectors Pak1, Pak4 and Rock activate LIMK1.17,21 Previous studies have shown that LIMK1 is closely related to the differentiation of gastric cancer, lymph node metastasis and TNM stage, and plays an important role in invasion and metastasis in gastric cancer. The gene discussed is PAK4; the disease is gastric cancer.