To determine whether WT1 is a regulator of E-cadherin gene expression in LNCaP and PC3 PCa cells, we transfected the cells with pCMV4 or GFP/WT1 expression vector and measured the effect of WT1 overexpression by TaqMan qRT-PCR as described below. The gene discussed is CDH1; the disease is posterior cortical atrophy.