A549 NSCLC cell line was cultured for 16 h under serum-starved conditions to dephosphorylate Akt and ERK1/2, and then treated with increasing concentrations (0.1–30 μM) of Sup ODNs or control ODNs for up to 24 h in normal medium. This evidence concerns the gene MAPK3 and non-small cell lung carcinoma.