To investigate whether ES-62 was suppressing the development of Th2 responses in OVA-induced airway inflammation by induction of regulatory T (Treg) cells, DLN tissue sections were stained for expression of the signature Treg, Th1 and Th2 transcription factors, Foxp3, Tbet and GATA3, respectively (Fig. 3F), and imaged and quantitatively analysed by LSC (Adams et al., 2004; Morton et al., 2007). This evidence concerns the gene TBX21 and inflammatory response.