CHAT and non-small cell lung carcinoma: To determine whether NSCLC cell lines express AChRs and ChAT, the mRNAs of five subtypes of mAChRs (M1–M5), three subtypes of nAChRs (α7, α9 and α10) and ChAT were examined by RT-PCR in human NSCLC cell lines H1299, H157, H460, A549 and human immortalized bronchi epithelial cell BEP2D.