In this study, we tried to develop IFN-γ gene therapy by a replication defective adenovirus encoding the human IFN-γ (Ad-IFNγ) on nasopharyngeal carcinoma (NPC), our results showed Ad-IFNγ effectively expressed in NPC cells, significantly inhibited tumor cell proliferation and induced cell apoptosis in vitro, inhibited the growth of xenografts in nude mice. Here, IFNG is linked to nasopharyngeal carcinoma.