The present data indicate that FSHD myotubes present clear changes in the relative abundance of proteins typical of caveolae (i.e., PTRF, MURC, and Gpi-anchor proteins such as THY1), which are membrane lipid microdomains that are enriched in cholesterol and glycosphingolipids and are often considered to be a specialized lipid raft subtype. This evidence concerns the gene CAVIN4 and facioscapulohumeral muscular dystrophy.