In conclusion, metabolic markers acquired with HR-MAS MR spectroscopy using CNB specimens showed significant correlation with histologic prognostic factors [ER, PR, HER2, triple negativity, histologic grade, Ki-67, and poor prognosis (detection of axillary lymph node metastasis, tumor size with a diameter >2 cm, or negative for ER or PR)]. This evidence concerns the gene ESR1 and neoplasm.