To examine whether or not CDF or anti-miR-21 inhibits the expression of CSC cell surface markers CD44 and EpCAM in CSC-like cells derived from PC cells, we conducted confocal imaging microscopy for assessing the expression of CD44 and EpCAM in the MiaPaCa-2 cells initiated tumor-derived sphere cells. This evidence concerns the gene EPCAM and pachyonychia congenita.