VEGFA and neoplasm: As shown in Figure 4C, MiaPaCa-2 tumor sphere cells produced relatively larger amount of VEGF under hypoxic conditions, compared to its parental MiaPaCa-2 cells (2174 pg/mL/104 MiaPaCa-2 tumor sphere cells vs 3248 pg/mL/106 MiaPaCa-2 cells; Figure 1C and 4C), suggesting that MiaPaCa-2 tumor spheres may promote angiogenesis by up-regulating the production of VEGF.