LCN2 and acute kidney injury: As analyzed by Western blotting, the sera from the mice and rabbits immunized with NGAL were able to recognize the recombinant NGAL protein (Figure 2A, sample 2, 4) and also specifically detected canine NGAL (25 kDa) from two renal failure dogs in which the presence of NGAL had been diagnosed by a commercial kit (Figure 2B, sample 2 and 3).