Consistent results were obtained using our NGAL ELISA with a higher OD being detected from the renal failure dogs (0.306 - 1.507; 306 pg/mL - 4492 pg/mL of recombinant NGAL protein) compared to the healthy group (0.114 - 0.356; 0 pg/mL - 314.3 pg/mL of recombinant NGAL protein; Figure 3A, right panel). The gene discussed is LCN2; the disease is Renal insufficiency.