The absence of a spatial correlation between upregulated expression of endothelial P-selectin, VCAM-1 and ICAM-1 and vascular sites of PMN accumulation in the tMCAO samples suggests the absence of the complete cascade of these events and that the mode of endothelial activation that occurs after ischemic stroke is not sufficient to trigger PMN extravasation into the brain parenchyma. This evidence concerns the gene ICAM1 and ischemic stroke.