In addition, the 1d2d-PSD-95-EGFP KI mice showed significantly increased anxiety-like behavior, as revealed by their shorter stay time in the light (Figure 6B, WT: 237.4 ± 7.9 s; KI: 110.8 ± 34.3 s; genotype effect, F(1,29) = 12.17; P = 0.0016, one-way ANOVA), fewer transitions between compartments (Figure 6C, WT: 42.2 ± 2.0 ; KI: 5.4 ± 1.6; genotype effect, F(1,29) = 209.2; P < 0.0001, one-way ANOVA), and longer latencies to enter the light side (Figure 6D, WT: 47.7 ± 7.0 s; KI: 211.1 ± 51.0 s; genotype effect, F(1,29) = 9.43; P = 0.0046, one-way ANOVA) in the light/dark transition test. This evidence concerns the gene DLG4 and Anxiety.