CREB3L1 and hepatocellular carcinoma: To analyze proteolytic activation of CREB3L1, we fractionated human hepatoma Huh7 cells (Nakabayashi et al., 1982) into membrane and nuclear fractions, and used an antibody reacting against the NH2-terminal domain of CREB3L1 (Denard et al., 2011) to examine the cleavage of CREB3L1 through immunoblot analysis.