In addition, to study the effect of this combination on the methylation status of breast cancer cells, we also examined the reactivation of expression of RAS-association domain family 1, isoform A (RASSF1A), a tumour suppressor gene pathway that can regulate proliferation, induce apoptosis, and bind to and stabilise microtubules [13]. The gene discussed is RASSF1; the disease is breast carcinoma.