In order to investigate any possible alteration in the expression of human Spry1 and Spry2 proteins in ovarian cancer, western blotting was also performed on the protein lysates obtained from the primary human ovarian cells HOSEpiC (Figure 1(a)) and quantitative comparison of the protein expression was carried out using ImageQuant software (Figures 1(b)–1(c)). Here, SPRY2 is linked to ovarian carcinoma.