SPRY1 and ovarian cancer: To determine subcellular distribution of Spry1 and Spry2 proteins in human epithelial ovarian cancer, we performed confocal immunofluorescence microscopy on ovarian cancer cell lines OVCAR-3 and SKOV-3, as well as human ovarian surface epithelial cell line HOSEpiC, using antibodies specific for Spry1 and Spry2.