The considerable beneficial effects of especially NO-10 group treatment (NO distillate administration at a dose of 375 μg/0.51 mL of distilled water/d) on glucose metabolism, insulin resistance, insulinotropic activity, leptin, dyslipidemia, liver enzymes, and PPARs may point out the insulin-like effect of NO and offer new approaches to treatment strategies that target both fat and glucose metabolism ultimately leadind to a reduction in both the chronic microvascular complications of type 2 diabetes and the risk of macrovascular events such as CVD. This evidence concerns the gene LEP and type 2 diabetes mellitus.