DIO3 and Anxiety: The relationship between hippocampal Dio3 expression, lower total Dio3, elevated T3 in hippocampus, and anxiety-related deficits was substantiated in a separate study using SB-BS reciprocal crosses, in which the SB difference in hippocampal Dio3 expression was exacerbated by exposure to prenatal alcohol, producing further elevation in hippocampal T3 levels and behavioral deficits (Sittig et al., 2011b).